Separation of Sulfur-containing Fatty Acids from Garlic, Allium Sativum, Using Serially Coupled Capillary Columns with Consecutive Nonpolar, Semipolar, and Polar Stationary Phases

Sulfolipids were originally discovered in diatom species, in 1966, by Morris Kates [1–3]. High levels of these sulfolipids have been found in the alga Ochromonas danica (Chrysophyceae, Chrysophyta) where they constitute 15% of total lipids and 3% of the dry weight of heterotrophically grown, stationary-phase cells [4,5]. More recently, sulfolipids have also been identified in more than 30 species of green (Chlorophyceae), brown (Phaeophyceae), and red (Rhodophyceae) freshwater and marine macrophytic algae [6–8] and other microalgal species [9–14]. The presence of sulfolipids in algae, diatoms, and marine invertebrates has been reported in several review articles [15–20].